Research Journal of Pharmacy and Technology

Jastria Pusmarani ¹, Mus Ifaya ¹, Muhammad Isrul ¹, Ahmad Saleh ¹, Himaniarwati ¹

Affiliations
1 Department of Pharmacy, STIKES Mandala Waluya, Kendari, ID

Abstract

Lannea coromandelica is one of plant dispersed in Sulawesi. Inhabitants from the Southeast Sulawesi apply Lannea coromandelica Bark to relief symptom of gastritis or peptic ulcer. Phenolic compounds of Lannea coromandelica Stem bark contains antioxidant activity. The study investigated the gastroprotective effect of Lannea coromandelica stem bark. Ethanol extract of Lannea coromandelica Stem bark (LCSB) was evaluated its anti-ulcer activity using aspirin-induced rats. Normal group was given 0.05% Na CMC. Positive group was given sucralfat 360 mg / kgBW and aspirin 600mg/kg BW, group I was given extract of LCSB at dose 400 mg / kgBW and aspirin 600 mg/kgBW, group II was given extract of LCSB at dose 800 mg / kg BW and aspirin 600 mg/kgBW, and group III was given extract of LCSB at dose 1200 mg / kg BW and aspirin 600 mg/kg BW. Extract of LCSB was administered orally in various doses for three days prior to the ulcer induction. On the 4^th-day rats, all rats were observed 8 hours after aspirin induction to determine ulcer index and percent inhibition. Results of this study revealed that LCSB extract significantly reduced the ulcer index and percent inhibition (p=0.00). Extract of LCSB shows gastroprotective activities.

Keywords

Bark of Javanesse wood, Lannea coromandelica, Aspirin, Gastroprotective, Rat.

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Muhammad Isrul ¹, Risky Juliansyah ¹, Ahmad Saleh ¹, Wa Ode Yuliastri ¹, Jastria Pusmarani ¹, Himaniarwati ¹, Wa Ode Wahyuni Maulidina ¹

Affiliations
1 Department of Pharmacy, STIKES Mandala Waluya Kendari, South-East Sulawesi, ID

Abstract

Last several years, researches about the plant as an alternative treatment of breast cancer have been developed. Curcuma aureginosa has been applied empirically as a breast cancer treatment and provide a satisfying result. The aim of this study is to examine cytotoxic activity of C.aureginosa extract to MCF-7 breast cancer cells, phytochemical analysis, and standardization of extract. Examination of breast cancer activity conducted by MTT method, extract was examined at concentration of 2000, 1000, 500, 250, 125, 62.5, 32.25, 15.625, and 7.8125 μg/ml, doxorubicin 1 μg/ml is applied as a positive control. Phytochemical Analysis conducted by GC-MS, and standardization of extract conducted based on Indonesia Pharmacopeia. The results reveal that extract has cytotoxic activity toward MCF-7 breast cancer cell. Concentration of 2000, 1000, 500, 250μg/ml has similar activity with doxorubicin 1 μg/ml. IC50 value of the extract is 76.24 μg/ml with 48 hours incubation. Standardization result determines extract profile and completes the standard of Indonesia Pharmacopeia. Phytochemical analysis result reveals Germacra-1(10), 4-diene-12-oic acid 6 alpha hydroxy gamma lactone (25.12%) and Cycloisolongifolene, 8, 9-dehydro-9-formyl- (21.61%) as a major compound. Cycloisolongifolene, 8, 9-dehydro-9-formyl-, Alpha-Humulene, Curcumene, and Curzerene assumed as cytotoxic toward MCF-7 breast cancer cell.

Keywords

Curcuma aureginosa, Breast Cancer, MCF-7, MTT, GC-MS, Standardization.

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